reference identifiers additional information antibody rabbit polyclonal anti giv girdin (Santa Cruz Biotechnology)

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Santa Cruz Biotechnology reference identifiers additional information antibody rabbit polyclonal anti giv girdin

Figure 1. <t>GIV</t> <t>(CCDC88A)</t> is highly expressed in spermatocytes in testis and localizes to the acrosomal cap. (A) Bar graph displays the relative fluorescence unit (RFU) of endogenous full-length GIV protein in immunoblots of organ lysates published previously using three independent anti-GIV antibodies raised against different epitopes of GIV (Anai et al., 2005). (Figure 1—source data 1)(B) RNA expression in the single-cell-type clusters identified in the human testis visualized by a UMAP plot (inset) and a bar plot. The bar plot shows RNA expression (pTPM) in each cell-type cluster.

Reference Identifiers Additional Information Antibody Rabbit Polyclonal Anti Giv Girdin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 18 article reviews

reference identifiers additional information antibody rabbit polyclonal anti giv girdin - by Bioz Stars, 2026-09

91/100 stars

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1) Product Images from "GIV/Girdin, a non-receptor modulator for Gαi/s, regulates spatiotemporal signaling during sperm capacitation and is required for male fertility"

Article Title: GIV/Girdin, a non-receptor modulator for Gαi/s, regulates spatiotemporal signaling during sperm capacitation and is required for male fertility

Journal: eLife

doi: 10.7554/elife.69160

Figure 1. GIV (CCDC88A) is highly expressed in spermatocytes in testis and localizes to the acrosomal cap. (A) Bar graph displays the relative fluorescence unit (RFU) of endogenous full-length GIV protein in immunoblots of organ lysates published previously using three independent anti-GIV antibodies raised against different epitopes of GIV (Anai et al., 2005). (Figure 1—source data 1)(B) RNA expression in the single-cell-type clusters identified in the human testis visualized by a UMAP plot (inset) and a bar plot. The bar plot shows RNA expression (pTPM) in each cell-type cluster.

Figure Legend Snippet: Figure 1. GIV (CCDC88A) is highly expressed in spermatocytes in testis and localizes to the acrosomal cap. (A) Bar graph displays the relative fluorescence unit (RFU) of endogenous full-length GIV protein in immunoblots of organ lysates published previously using three independent anti-GIV antibodies raised against different epitopes of GIV (Anai et al., 2005). (Figure 1—source data 1)(B) RNA expression in the single-cell-type clusters identified in the human testis visualized by a UMAP plot (inset) and a bar plot. The bar plot shows RNA expression (pTPM) in each cell-type cluster.

Techniques Used: Fluorescence, Western Blot, RNA Expression

Figure 2. Transcripts of CCDC88A (GIV) are downregulated in infertile male testis and semen. (A) Schematic displays the approach used to search NCBI GEO database for testis and sperm transcriptomic datasets suitable to study correlations between the abundance of CCDC88A transcripts and male fertility. (B–E) Whisker plots show the relative abundance of CCDC88A (expressed as Log2 normalized expression; see Materials and methods for different normalization approaches used for microarray and RNA-seq datasets) in sperm or testis samples (as annotated using schematics) in samples

Figure Legend Snippet: Figure 2. Transcripts of CCDC88A (GIV) are downregulated in infertile male testis and semen. (A) Schematic displays the approach used to search NCBI GEO database for testis and sperm transcriptomic datasets suitable to study correlations between the abundance of CCDC88A transcripts and male fertility. (B–E) Whisker plots show the relative abundance of CCDC88A (expressed as Log2 normalized expression; see Materials and methods for different normalization approaches used for microarray and RNA-seq datasets) in sperm or testis samples (as annotated using schematics) in samples

Techniques Used: Whisker Assay, Expressing, Microarray, RNA Sequencing

Figure 8. Summary and working model: spatiotemporally segregated roles of GIV/Girdin during sperm capacitation. Schematic summarizes the key findings in this work and places them in the context of existing literature. GIV is likely to primarily function during capacitation of sperm, during which it fulfills two key roles as a signal transducer in a spatiotemporally segregated manner. The first role (right, top) is in the head of the sperm, where GIV’s GEM motif inhibits the AC→cAMP pathway and prevents acrosomal reaction. The second role (right, bottom) is in the mid-piece and tail region of the sperm, which involves tyrosine phosphorylation of GIV, which

Figure Legend Snippet: Figure 8. Summary and working model: spatiotemporally segregated roles of GIV/Girdin during sperm capacitation. Schematic summarizes the key findings in this work and places them in the context of existing literature. GIV is likely to primarily function during capacitation of sperm, during which it fulfills two key roles as a signal transducer in a spatiotemporally segregated manner. The first role (right, top) is in the head of the sperm, where GIV’s GEM motif inhibits the AC→cAMP pathway and prevents acrosomal reaction. The second role (right, bottom) is in the mid-piece and tail region of the sperm, which involves tyrosine phosphorylation of GIV, which

Techniques Used: Phospho-proteomics

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Article Title: GIV/Girdin, a non-receptor modulator for Gαi/s, regulates spatiotemporal signaling during sperm capacitation and is required for male fertility
Article Snippet: .. Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Rabbit polyclonal anti- GIV (Girdin) (T- 13) Santa Cruz Biotechnology sc- 133371 Antibody Rabbit monoclonal diagnostic grade anti- Girdin/GIV antibody Custom; Sprint Bioscience SP173 Validated in prior publication Ghosh et al., 2016 Antibody Rabbit polyclonal anti- GIV (Girdin) (CC- Ab) Millipore Sigma ABT80 Antibody Rabbit polyclonal anti- GIV pS1675 Ab Custom, from 21t Century Biosciences n/a Validated in prior publication Bhandari et al., 2015 Antibody Rabbit polyclonal anti- GIV pS1689 Ab Custom, from 21st Century Biosciences n/a Validated in prior publication LópezSánchez et al., 2013 Antibody Rabbit monoclonal anti- GIV pY1764 Ab Custom, Spring Biosciences Inc n/a Validated in prior publications Midde et al., 2015; Lin et al., 2011; Midde et al., 2018; Dunkel et al., 2016 Antibody Rabbit monoclonal antipT308 AKT Cell Signaling Technology D9E Antibody Mouse monoclonal anti- total AKT Cell Signaling Technology 40D4 Antibody Mouse anti- sp56 Thermo Fisher Scientific (Waltham, MA) MA1- 10866 Antibody Mouse anti- human hexokinase 1/2 monoclonal antibody R&D Systems, (Minneapolis, MN) MAB8179 Antibody Rabbit anti- phospho- PKA substrate (RRXS*/T*)100G7E Cell Signaling Technology 9624 Antibody Goat anti- rabbit IgG, Alexa Fluor 594 conjugated ThermoFisher Scientific A11072 For immunofluorescence (IF) Antibody Goat anti- mouse IgG, Alexa Fluor 488 conjugated ThermoFisher Scientific A11017 For immunofluorescence (IF) Antibody IRDye 800CW goat antimouse IgG secondary (1:10,000) LI- COR Biosciences 926- 32210 For immunoblotting Antibody IRDye 680RD goat anti- rabbit IgG secondary (1:10,000) LI- COR Biosciences 926- 68071 For immunoblotting Reynoso, Castillo, Katkar et al. eLife 2021;10:e69160. .. DOI: https://doi.org/10.7554/eLife.69160 19 of 30 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain, strain background (Mus musculus) UbcCre- Ert2/+ xCcdc88afl/fl and Ccdc88afl/fl mice Masahide Takahashi (Nagoya University Graduate School of Medicine, Nagoya, Japan) n/a Strain, strain background (Mus musculus) WT and GIV- cKO (conditional KO) mice This work UbcCre- Ert2/+ xCcdc88afl/fl (experimental group), and UbcCre- Ert2/ Cre- Ert2 x Ccdc88afl/fl (control group) This work; male Strain, strain background (Mus musculus) C57BL/6 J mice (male and female) The Jackson Laboratory Stock number: 000664; Bar Harbor, ME Male: source of sperm for biochemical, immunohistochemical, peptide transduction, and functional assays Female: for co- housing studies; source for eggs for IVF assays Chemical compound Paraformaldehyde 16% Electron Microscopy Biosciences 15710 Chemical compound MTT Millipore Sigma 475989- 1 GM Other DAPI (4',6- Diamidino- 2- Phenylindole, Dilactate) Thermo Fisher Scientific D3571 Used in IF studies for staining DNA/ nucleus Kit/reagent HisPurä Cobalt Resin Thermo Scientific 89964 Kit/reagent Glutathione Sepharoseâ 4B Sigma- Aldrich GE17- 0756- 04 Chemical compound Protease inhibitor cocktail Roche 11873580001 Chemical compound Tyr phosphatase inhibitor cocktail Sigma- Aldrich P5726 Chemical compound Ser/Thr phosphatase inhibitor cocktail Sigma- Aldrich P0044 Other PVDF Transfer Membrane, 0.45 mM Thermo Scientific 88518 Used for transfer in immunoblots Commercial assay or kit Countess II Automated Cell Counter Thermo Fisher Scientific AMQAX1000 Commercial assay or kit Leica TCS SPE Confocal Leica Microsystems TCS SPE Commercial assay or kit Light Microscope (brightfield images) Carl Zeiss LLC Axio Observer, Inverted; 491917- 0001- 000 Software ImageJ National Institute of Health https://imagej.net/Welcome Software Prism GraphPad https://www.graphpad.com/ scientific-software/prism/ Software LAS- X Leica https://www.leicamicrosystems.com/ products/microscopesoftware/p/leica-las-x-ls Software Illustrator Adobe https://www.adobe.com/ products/illustrator.html Software ImageStudio Lite LI- COR https://www.licor.com/bio/ image-studio-lite/ Continued Contact for reagent and resource sharing: Pradipta Ghosh ( prghosh@ ucsd. edu).

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reference identifiers additional information antibody rabbit polyclonal anti giv girdin (Santa Cruz Biotechnology)

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1) Product Images from "GIV/Girdin, a non-receptor modulator for Gαi/s, regulates spatiotemporal signaling during sperm capacitation and is required for male fertility"

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